Classification

Definition

Examples

B

“Animals being bred, conditioned, or held for use in teaching, testing, experiments, research, or surgery, but not yet used for such purposes.” 

·       Studies in which only breeding occurs (parents + offspring) including animals that will be culled and not used in experiments;

·          Wild animals observed in their natural habitat.

C

“Animals upon which teaching, research, experiments, or tests will be conducted involving no pain, distress, or use of pain-relieving drugs.”

·          Euthanasia and tissue procurement from euthanized animals (if euthanasia method meets recommendations of AVMA Panel);

·          Grow tumor in animal & end study before tumor gets large enough to make animal sick;

·          Routine injections and adminstration of oral medications;

·          Collect blood  from peripheral vein;

·          Catheterization of a peripheral vein;

·          Standard Radiography;

·          Non-invasive imaging (PET/MRI) in an anesthetized animal

D

“Animals upon which experiments, teaching, research, surgery, or tests will be conducted involving accompanying pain or distress to the animals and for which appropriate anesthetic, analgesic, or tranquilizing drugs will be used.” 

·          Non-survival surgery performed on anesthetized animals;

·          Perfusion or tissue procurement from anesthetized animas prior to death

·          Survival surgery on anesthetized animals performed by trained personnel + post-op analgesics are given;

·          Blood collection by orbital sinus puncture or other invasive route that requires anesthesia for collection;

·          Tumor study on which animal develops cancer, but study ends at a defined point before the animal begins to experience demise from the cancer;

·          Immunization studies done using LARC Immunological procedures guidelines;

·          Endoscopic surgery;

·          Animal is injected with a chemical, toxin, rDNA or infectious agent, but the study duration and endpoints prevent significant illness;

·          Animal model develops a genetic disease, but disease progression will be monitored & demise prevented by appropriate euthanasia;

·          Animal will develop an experimental disease, but progression of disease will be monitored and animal will be removed from the study prior to suffering significant ill effects from disease. Studies;

·          Using  food or fluid restriction in which animals do not develop dehydration.

E

“Animals upon which teaching, experiments, research, surgery, or tests will be conducted involving accompanying pain or distress to the animals and for which the use of appropriate anesthetic, analgesic, or tranquilizing drugs will adversely affect the procedures, results or interpretation of the teach, research, experiments, surgery, or tests.” 

·          Pain research studies in which no analgesics are used;

·          Studies which have death of the animal as a data point for any groups (control or experimental);

·          Immunization protocols using footpad injections;

·          Tumor study on which animal develops cachexia & other physical signs of systemic cancer;

·          Tumor studies using metastatic cell lines—metastasis is the focus of the study;

·          Studies using  food or fluid deprivation that will produce dehydration.or produce an altered physiological or biochemical state in the test subject.

 For Category E studies, the PI must fill out Appendix E.  Be advised that this information may be forwarded to USDA.  The IUSM IACUC requires that a pilot study be conducted prior to use of death as an experimental data point