Tentative Lecture Schedule

6/8 Mon	8:30 10:30 11:30 13:30	Introduction, DNA and RNA structure (Lee) DNA replication and growing E. coli for plasmid isolation (Lee) Transcription (Lee) Genomic DNA isolation and translation (Lee)
6/9 Tue	8:30 10:30 11:30	Translation (2hrs, Lee) Plasmid DNA isolation (Konger) Enzymes used in molecular biology (Lee)
6/10 Wed	8:30 10:30 11:30	Enzymes used in molecular biology (2 hrs, Lee) Promoters and operons (Lee) Gel electrophoresis (Lee)
6/11 Thr	8:30 9:30 10:30 11:30	Total and Poly-A RNA isolation (Murrell) Recover DNA from gels, Spin dialysis, DNA concentration determination (Lee) Cloning vectors (Lee) Ligation, transformation, and screening of recombinant plasmids (Lee)
6/12 Fri	8:30 9:30 10:30	Cloning strategies (Lee) E. coli genotypes (Lee) DNA labeling, DNA and RNA probes (2hrs, Lee)
6/15 Mon	8:30 9:30 10:30 11;30	Southern and Northern (Lee) Hybridization (Lee) Life cycle of lambda phage (Lee) cDNA synthesis and cloning (Lee)
6/16 Tue	8:30 9:30 10:30	Lambda packaging and cDNA library screening (Lee) Restriction mapping (Lee) DNA sequencing (2hrs, Lee)
6/17 Wed	8:30 9:30 10:30 11;30	Biology of M13 (Lee) Phagemid and in vivo excision of pBluescript from lambda ZAP (Lee) EMBL 3, EMBL4, Cosmid, YAC, BAC, PAC (Lee) Proteomics (Wang)
6/18 Thr	8:30 9:30	DNA sequence analyses (Liu) PCR theory and applications (3 hrs, Lee)
6/19 Fri	8:30 10:30 11:30	PCR theory and applications (2 hrs, Lee) Functional genomics (Xuei) Single nucleotide polymorphism (Xuei)
6/22 Mon	8:30 10:30 11:30	Expression of foreign genes in E. coli (2 hrs, Lee) Expression of foreign genes in eukaryotic expression systems (Vidal) Gateway expression systems (Lee)
6/23 Tue	8:30 9:30 11:30	Fluorescent in situ hybridization (Thurston) Mutagenesis and homologous recombination (Lee) Viral vectors for gene transfer (2 hrs, Lee)
6/24 Wed	8:30 9:30 10:30 11:30	Molecular Genetics (Dlouhy) Molecular diagnostics (Blue) Transgenic animals and gene knockouts (Field) Clinical applications of gene transfer technologies (Cornetta)
6/25 Thr	8:30 9:30 10:30 11:30	siRNA and ET/Red cloning system (Lee) Transfection, reporter assays, gel shift, footprinting, 2-hybrid (Lee) Primer extension, S1, RNaseA protection, SSCP, RAPD, AFLP (Lee) mRNA differential display, DNA microarrays, TOGA, SAGE, MPSS, SOLiD, RDA (Lee)

Tentative Lab Schedule

	June 8 Mon	June 9 Tue	June 10 Wed	June 11 Thr	June 12 Fri
Plasmid DNA	1 ml-10 ml 10 ml-1 liter amplify (p10) Steps 2-4	Lyse cells Isolate DNA (p10-11) Steps 5-18	CsCl spin (p11-12) Steps 19-28	Purify band Dialyze (p12-13) Steps 29-37	Determine conc. Run gel Steps 38-40
Cloning			Cut pMDM2 & vectors (p18) Electrophoresis (p19-20)	Extract insert Ligate (p19-28)	Transform (p28-31)
Genomic DNA Southern	Lyse cells Prot. K (p44) Steps 1-4	Extract DNA (p44-45) Steps 5-12	Purify & cut DNA (p45) Steps 13-16	Run gel (p45) Steps 17-18	Process gel & transfer (p46)
Northern				Isolate RNA (p53) Steps 1-12	Purify RNA (p53) Steps 13-17

	June 15 Mon	June 16 Tue	June 17 Wed	June 18 Thr	June 19 Fri
Cloning	pACYC-rep. plate pTZ-pick, grow pBR-rep. Plate	Grow Screen Colony hyb.	Screen (p32-36) Map (p32-36) Hyb. (p32-36)	Map Wash Filters	Develop film
Southern	Bake membrane (p46)	Prehyb. (p48)	Hyb. (p48)	Wash, ARG (p48)	Develop film
Northern	Run gel (p55) Transfer (p56)	Bake Prehyb. (p57)	Make probe hyb. (p57)	Wash; ARG (p57)	Develop film
PCR/TOPO Cloning			Isolate template (p61-62)	PCR (p62-63)	Ligate Transform (p66-67)
PCR/RFLP					Spin dialyze DdeI digestion Run gel (p65)
PCR/Dot-blot					Denature Dot-blot (p63-64) Bake membrane

	June 22 Mon	June 23 Tue	June 24 Wed	June 25 Thr
PCR/Dot-blot	Prehyb. (p64)	Hyb. (p64)	Wash (p64), ARG (p64)	Read results
Real-time PCR		Induce iNOS, total RNA isolation, reverse transcription (p71-72)	Setup, Temp. cycling Interpretation (p72-73)