Molecular Biology Workshop

Indiana University School of Medicine

Introduction	Course Content	Course Director
Workshop Dates	Tentative Lecture and Lab Schedule	Participant comments

Introduction

This workshop is a combination of lectures and hands-on laboratories in Molecular Biology. It is a 3-credit graduate course entitled "Methods in Molecular Biology and Pathology" (IUPUI course number G890). It is also open to faculty and staff members of Indiana University. Individuals from other institutions and industries are also accepted if space permits. This course is usually offered in the summer (Summer Section I or II). Students who would like to take the course for credits are to register through the IUPUI on-line registration systems. In order for you to be able to register for a credit class at IUPUI, you need to be enrolled as a student. For instructions to enroll to take graduate courses, please visit the Graduate Office website at: http://www.iupui.edu/~gradoff/office, or contact Sue Wheeler in the Graduate Office at sulwheel@iupui.edu or 274-1861 for more information. Other individuals sign up the course by contacting Dr. C. H. Lee by phone (317-274-2596) or by E-mail (chlee@iupui.edu).

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Course Information

Section: 2012 Summer Section I
Department: GRAD
Course number: G890
Class number: 10231
Open registration dates: Mar 31 - May 11

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Course Content

Lectures:

DNA and RNA structure
Replication
Transcription
Translation
mRNA processing
Enzymes used in molecular biology
Construction of cDNA and genomic libraries
cDNA library screening
Conventional and pulsed-field gel electrophoresis
Promoters and operons
Cloning vectors
Cloning strategies
Restriction mapping
DNA sequencing
Polymerase chain reaction
Lambda phage replication and transcription
Site-directed mutagenesis
Homologous recombination
RNA interference and applications of siRNA
Transfection
CAT assay
Luciferase assay
Expression of foreign genes in E. coli
Expression of foreign genes in eukaryotic cells
mRNA differential display
S1 nuclease analysis
Random amplification of polymorphic DNA
Primer extension
Gel shift (gel retardation) assay
Single-strand conformation polymorphism
Amplified fragment length polymorphism
DNaseI footprinting
Yeast two-hybrid system
Fluorescent in situ hybridization
Gene targeting and gene therapy
Single nucleotide polymorphism
Functional genomics and applications of DNA microarrays
Proteomics
Next generation DNA sequencing
Epigeneticc

Labs:

Isolation of plasmid DNA from bacteria
Isolation of genomic DNA from mammalian cells
Total cellular RNA isolation
Southern hybridization
Northern hybridization
Dot-blot hybridization
Cloning of DNA fragments
Identification of recombinant clones
Restriction mapping
Polymerase chain reaction
Cloning of PCR products
Restriction fragment length polymorphism
Real-time PCR

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2012 Workshop

Place: Lab: IUPUI Fesler Hall (1120 South Dr., Indianapolis, In 46202), 4th floors; lecture: Fesler Hall, 3rd floor
Dates: June 4 - 21, 2012 (Monday through Friday)
Lecture time: 8:30 A.M. - 12: 30 P.M. except June 4 (8:30 A.M. - 2: 30 P.M.)
Lab time: 1:30 P.M. - 5:00 P.M. (approximately)
Final exam: June 25, 8:30 A.M. - 12:00 noon (For those who take this course for credit)

Participant comments

This is an excellent course. I will recommend it to any of my colleagues interested in learning molecular biology. It is a good summer course. Thank you for a job well done.
Excellent, excellent, excellent! I’ve already recommended the course to many people.
I think that this course was very informative and is one of the best courses I’ve taken at IUPUI.
Very, very good! This course helps me understand a lot of concepts of molecular biology.
Overall, it was a decent overview of molecular biology.
Excellent for scientists who want to pursue biotech research.
This was a good course and will help my interactions with customers and other scientists at work. I will offer a positive recommendation of this course to colleagues who lack a biology background but must work with biologists on projects.
Very useful!
A++! I would recommend it highly to others.
Excellent course.
A very useful course.
Excellent! I don’t mind to take it again. I’ve learned a lot. Very productive three weeks.
A great course!
Will be very useful! Good for anyone interested in molecular biology.
This course is very informative and I will definitely recommend it to others.
Very helpful for future lab work!
Excellent! Very informative.
A+! I liked it a lot.
A very good overview and direction on molecular biology theories and techniques.
Overall this course is WONDERFUL because it exposes students to the techniques and concepts of molecular biology. I learned a lot and enjoyed the course.
Excellent course. Very pleased with what I’ve learned. Would recommend it to others.
I really enjoyed it and learned a lot. I’m glad I took this course and will recommend it to others.
Excellent! Excellent! Excellent! I would highly recommend it.
It is a good course and it is extremely well managed and directed.
Very educational. A fun learning experience!
This is a high quality course and provided a good review and updates.
Excellent! I learned much and enjoyed the course.
Very useful and good. I learned a lot from it.
A+! Excellent!
Overall, I think the course was excellent. I would recommend this to anyone interested in molecular biology.
I’m glad I was part of the workshop. It really helped me to understand molecular biology. I would recommend it to anyone who wants to do it in the future.
Very good! Thank you to everyone who put this together and make it work.

This webpage was created by Chao-Hung Lee on December 27, 1999
Last revision: January 16, 2012
URL: http://www.iupui.edu/~mbwkshp/