Barbara Kluve-Beckerman, Ph.D., Associate Scientist.

Tel: (lab) (317) 278-3439 
Fax:        (317) 278-3429 
Email 

EDUCATION:

Wittenberg University B.A. Biology 1973

University of Cincinnati M.S. Biology 1976

Case Western Reserve University Ph.D. Biochemistry 1981 

ACADEMIC APPOINTMENTS:

1981-83 Postdoctoral Fellow, NIH, National Heart, Lung, Blood Institute, Bethesda, MD (Ronald G. Crystal, M.D.)

1983-87 Postdoctoral Fellow, Department of Medicine, Indiana University, Indianapolis, IN (Merrill D. Benson, M.D.)

1987-97 Assistant Scientist, Department of Medicine, Indiana University, Indianapolis, IN

1997-98 Assistant Scientist, Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN

1998-99 Associate Scientist, Department of Medical and Molecular Genetics, Indiana University, Indianapolis, IN

1999 - Associate Scientist, Department of Pathology and Laboratory Medicine, Indiana University, Indianapolis, IN 

PROFESSIONAL ORGANIZATIONS:

International Society of Amyloidosis 

HONORS AND AWARDS:

Wittenberg University Alumni Scholarship Women's Honor Society, Wittenberg University 

SERVICE:

Admissions Committee, Department of Medical & Molecular Genetics (current). 
Medical Student Research Program (Committee member). 

PROFESSIONAL ACTIVITIES:

Served as co-chair of the session AProtein AA/SAA and Secondary Amyloidosis@ at the International Symposium on Amyloidosis, Oslo, Norway, 1990.

Selected for oral presentations at the International Symposia on Amyloidosis, Hakone, Japan (1987); Oslo, Norway (1990); Kingston, Ontario, Canada (1993); Mayo Clinic, Rochester, MN (1998).

Invited speaker at the American College of Rheumatology Continuing Medical Education Topical Symposium: Amyloidosis, New Orleans, LA, 1992.

Invited speaker at the Gordon Conference: Serum Amyloid A and Atherogenesis, Ventura, CA, 1996 and Henniker, NH, 1998.

Reviewed manuscripts for :"Biochemical and Biophysical Acta, The Journal of Immunology, The Journal of Rheumatology, and The Scandinavian Journal of Immunology".

Serving as an editor of the journal  Amyloid. The International Journal of Experimental and Clinical Investigation (1995-present).

Reviewer of National Research Service Award (NRSA) applications - 1998.

Served as Opponent at the dissertation defense of Cathrine Foyn Bruun, M.D., Department of Pediatrics, Institute of Cinical Medicine, University of Tromso, Norway. 

GRANTS AND FELLOWSHIPS:

National Research Service Award (Postdoctoral Fellowship), 1981 - 1984.

Arthritis Foundation Postdoctoral Fellowship, 1984 - 1986.

NIH (P60 AR20582): Multipurpose Arthritis Center Grant, 09/01/86 - 11/30/92, Molecular Biology Core Director.

NIH (P60 AR20582): Multipurpose Arthritis Center Grant, 09/01/86 -11/30/89, Principal Investigator of Development and Feasibility

NIH (P60 AR20582): Multipurpose Arthritis Center Grant, 12/01/89 -11/30/91, Principal Investigator of Development and Feasibility Study

NIH (R29 DK42657): Differential Regulation of Human Serum Amyloid A Isotypes

06/01/90 - 05/31/95, Principal Investigator.

NIH (RO1 DK49730): Metabolism of Serum Amyloid A (SAA:HDL),

06/01/95 - 05/31/99, Principal Investigator. 

PUBLICATIONS:

1. Kluve, B., Merrick, W.C., Stanbridge, E., and Gershman, H.: Mycoplasmas induce collagenase in BALB/c 3T3 cells. Nature 292:855-857, 1981.

2. Kluve, B., Merrick, W.C., and Gershman, H.: Mycoplasma- induced BALB/c 3T3 collagenase is a mammalian enzyme. Biochem. J. 212:641-647, 1983.

3. Wallace, M.R., Naylor, S.L., Kluve-Beckerman, B., Long, G.L., McDonald, L., Shows, T.B., and Benson, M.D.: Localization of the human prealbumin gene to chromosome 18. Biochem. Biophys. Res. Commun. 129:753-758, 1985.

4. Miskulin, M., Dalgleish, R., Kluve-Beckerman, B., Rennard, S., Tolstoshev, P., Brantly, M., and Crystal, R.G.: Human type III collagen gene expression is coordinately modulated with the type I collagen genes during fibroblast growth. Biochemistry 25:1408-1413, 1986.

5. Kluve-Beckerman, B., Naylor, S.L., Marshall, A., Gardner, J.C., Shows, T.B., and Benson, M.D.: Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms. Biochem. Biophys. Res. Commun. 137:1196-1204, 1986.

6. Kluve-Beckerman, B., Long, G.L., and Benson, M.D.: DNA sequence evidence for polymorphic forms of human serum amyloid A (SAA). Biochem. Genetics 24:795-803, 1986.

7. Kluve-Beckerman, B., Dwulet, F.E., and Benson, M.D.: Human serum amyloid A: Three hepatic mRNAs and the corresponding proteins in one person. J. Clin. Invest. 82:1670-1675, 1988.

8. Harats, N., Kluve-Beckerman, B., Skinner, M., Passo, M. Quinn, L., and Benson, M.D.: Lack of association of a restriction fragment length polymorphism for serum amyloid P gene with reactive amyloidosis. Arthritis Rheum. 32:1325- 1327, 1989.

9. Brinckerhoff, C.E., Mitchell, T.I., Karmilowicz,M.L., Kluve-Beckerman, B., and Benson, M.D.: Autocrine induction of collagenase in fibroblasts by serum amyloid-like and b2microglobulin-like proteins. Science 243:655-657, 1989.

10. Kluve-Beckerman, B., Dwulet, F.E., DiBartola, S.P., and Benson, M.D.: Primary structures of dog and cat amyloid A proteins: Comparison to human AA. Comp. Biochem. Physiol. 94B:175-183, 1989.

11. Kluve-Beckerman, B., Drumm, M., and Benson, M.D.: Non-expression of the human serum amyloid A three (SAA3) gene. DNA and Cell Biology 10:651-661, 1991.

12. Liepnieks, J.J., Dwulet, F.E., Benson, M.D., Kluve-Beckerman, B., and Kushner, I.: The primary structure of serum amyloid A protein in the rabbit: Comparison with serum amyloid A proteins in other species. J. Lab. Clin. Med. 118:570-575, 1991.

13. Kluve-Beckerman, B., Malle, E., Vitt, H., Pfeiffer, Benson, M., and Steinmetz, A.: Characterization of an isoelectric focusing variant of SAA1 (ASP-72) in a family of Turkish origin. Biochem. Biophys. Res. Commun. 181:1107-1102, 1991.

14. Aldo-Benson, M. Kluve-Beckerman, B., Hardwick, J., and Lockwood, M.: Ethanol inhibits production of messenger ribonucleic acid for k-chain in stimulated B lymphocytes. J. Lab. Clin. Med. 119:32-37, 1992.

15. Stevens, G., Ramsay, M., Kluve-Beckerman, B., and Jenkins, T.: A new Negroid-specific HindIII polymorphism in the serum amyloid A1 (SAA1) gene increases the usefulness of the SAA locus in linkage studies. Genomics 15:242-243, 1993.

16. Fife, R.S., Kluve-Beckerman, B., Houser, D.S., Proctor, C., Liepnieks, J., Masuda, I., McCarty, D.J., and Ryan, L.M.: Evidence that a 550,000-dalton cartilage matrix glycoprotein is a chondrocyte membrane-associated protein closely related to ceruloplasmin. J. Biol. Chem. 268:4407-4411, 1993.

17. Kluve-Beckerman, B., Song, M., Benson, M.D., and Liepnieks, J.J.: Recombinant murine serum amyloid A from baculovirus-infected insect cells: Purification and characterization. Biochim. Biophys Acta 1182:303-310, 1993.

18. Yamada, T., Kluve-Beckerman, B., Liepnieks, J.J., and Benson, M.D.: Fibril formation from recombinant human serum amyloid A. Biochim. Biophys. Acta 1226:323-329, 1994.

19. Yamada, T., Kluve-Beckerman, B., Kuster, W.M., Liepnieks, J.J., and Benson, M.D.: Measurement of serum amyloid A4 (SAA4), its constitutive presence in serum. Amyloid: Int. J. Exp. Clin. Invest. 1:114-118, 1994.

20. Liepnieks, J.J., Kluve-Beckerman, B., and Benson, M.D.: Characterization of amyloid A protein in human secondary amyloidosis: The predominant deposition of serum amyloid A1. Biochim. Biophys. Acta. 1270:81-86, 1995.

21. Yamada, T., Liepnieks, J.J., Kluve-Bekcerman, B., and Benson, M.D.: Cathepsin B generates the most common form of amyloid A (76 residues) as a degrada-tion product from serum amyloid A. Scand. J. Immunol. 41:91-97, 1995.

22. Yamada, T., Kluve-Beckerman, B., Liepnieks, J.J., and Benson, M.D.: In vitro degradation of serum amyloid A by cathepsin D and other acid proteases. Possible protection against amyloid fibril formation. Scand. J. Immunol. 41:570-574, 1995.

23. Kluve-Beckerman, B., and Song, M.: Genes encoding human serum amyloid A proteins SAA1 and SAA2 are located 18 kb apart in opposite transcriptional orientations. Gene 159:289-290, 1995.

24. Yamada, T., Liepnieks, J., Benson, M.D., and Kluve-Beckerman, B.: Accelerated amyloid deposition in mice treated with the aspartic protease inhibitor, pepstatin. J. Immunol. 157:901-907, 1996.

25. Kluve-Beckerman, B., Yamada, T., Hardwick, J., Liepnieks, J.J., and Benson, M.D.: Differential plasma clearance of murine acute phase serum amyloid A proteins, SAA1 and SAA2. Biochem. J. 322:663-669, 1997.

26. Kluve-Beckerman, B., Hardwick, J., and Liepnieks, J.J.: Direct evidence that serum amyloid A (SAA):HDL interaction requires the amino-terminal portion of SAA. (Submitted).

27. Kluve-Beckerman, B., Liepnieks, J.J., Wang, L. and Benson, M.D.: A cell culture system for the study of amyloid pathogenesis: Amyloid formation by murine peritoneal cells in the presence of recombinant serum amyloid A. Am. J. Pathol. 155:123-133, 1999.

28. Wang, L., Kluve-Beckerman, B., Takasugi, K., Uemichi, T., Yamada, T., Nakamura, M., and Benson, M.D.: Human serum amyloid A1 allele frequencies in American Caucasian and Japanese populations. (Submitted).

29. Wang, L., Liepnieks, J. J., Benson, M. D. and Kluve-Beckerman, B.,: Expression of SAA and amyloidogenesis in congenic mice of CE/J and C57BL/6 strains. Amyloid: Int. J. Exp. Clin. Invest., in press.

30. Orpiszewski, J., Schormann, N., Kluve-Beckerman, B., Liepnieks, J. J., and Benson, M. D.: Protein aging hypothesis of Alzheimer disease. Submitted. 

ABSTRACTS:

1a. Kluve-Beckerman, B. and Benson, M.D.: In vitro translation of human serum amyloid A. Fed. Proc. 43:1928, 1984.

2a. Wallace, M.R., Kluve-Beckerman, B., Long, G.L., Conneally, P.M., and Benson, M.D.: Cloning and sequencing of human prealbumin cDNA: Application to diagnosis of hereditary amyloidosis. Clin. Res. 33:592A, 1985.

3a. Kluve-Beckerman, B., Long, G.L., and Benson, M.D.: Cloning and nucleotide sequence of human SAA-specific cDNAs. Clin. Res. 34:618A, 1986.

4a. Harats, N., Kluve-Beckerman, B., Passo, M., Quinn, L., and Benson, M.D.: Frequency of SAP gene polymorphism in secondary amyloidosis. Am. J. Hum. Genet. 43:a85, 1988.

5a. Kluve-Beckerman, B., Benson, II, M.D. and Benson, M.D.: Human serum amyloid A: A third gene. Arthritis Rheum. 32:D109, 1989.

6a. Kluve-Beckerman, B. and Benson, M.D.: The human SAA gene family. VIth International Symposium on Amyloidosis, Oslo, Norway 1990.

7a. Kluve-Beckerman, B., Fortney, K. and Benson, M.D.: Human SAA3 cannot be involved in the pathogenesis of rheumatoid arthritis. Arthritis Rheum., 1991.

8a. Kluve-Beckerman, B., Song, M., Benson, M.D., and Liepnieks, J.J.: Baculovirus-infected insect cells as a source of isotypically pure, radiolabeled murine SAA1 and SAA2. VIIth International Symposium on Amyloidosis, Kingston, Ontario, Canada, July 1993.

9a. Yamada, T., Kluve-Beckerman, B., Benson, M.D., and Liepnieks, J.J.: Efficient production of recombinant human serum amyloid A proteins. VIIth International Symposium on Amyloidosis, Kingston, Ontario, Canada, July 1993.

10a. Kluve-Beckerman, B., Liepnieks, J.J., Wang, L., and Benson, M.D.: Amyloid deposition in cultures of murine peritoneal cells incubated with recombinant serum amyloid A. VIII International Symposium on Amyloidosis, The Mayo Clinic, Rochester, MN, August 1998.

11a. Kluve-Beckerman, B., Hardwick, J., Mercer, N., and Liepnieks, J.J.: Direct evidence that the amino-terminal portion of serum amyloid A (SAA) is required for binding to HDL. VIII International Symposium on Amyloidosis, The Mayo Clinic, Rochester, MN, August 1998.

12a. Nakamura, M., Ando, Y., Kluve-Beckerman, B., Ohlsson, P.-I., Hamidi Asl, K., Liepnieks, J.J., and Benson, M.D.: Dual expression of normal TTR and Met30 TTR using baculovirus system. VIII International Symposium on Amyloidosis, The Mayo Clinic, Rochester, MN, August 1998.

13a. Wang, L., Kluve-Beckerman, B., Nakamura, M., Benson, M.D., Takasugi, K., Yamada, T., and Uemichi, T.: Human serum amyloid A1 alleles frequencies in American Caucasian and Japanese populations. VIII International Symposium on Amyloidosis, The Mayo Clinic, Rochester, MN, August 1998.

14a. Liepnieks, J.J., Reginato, A.J. Eid, H., Kastner, D.L., Ansentijevich, I., Kluve-Beckerman, B., and Benson, M.D.: Liver amyloid in a kindred with familial recurrent fever is derived predominantly from SAA2. VIII International Symposium on Amyloidosis, The Mayo Clinic, Rochester, MN, August 1998. 

BOOKS:

1b. Benson, M.D., Dwulet, F.E., Kluve-Beckerman, B., and Aldo-Benson, M.: Structure and Function of SAA. InMarker Proteins in Inflammation, Ed. by Laurent, P., Grimaud, J.A. and Bienvenu, J., Vol.3, Walter de Gruyter, Berlin, New York, 1985.

2b. Kluve-Beckerman, B., Benson, M.D., Dwulet, F.E., DiBartola, S.P., and Benson, M.D.: Phylogenetic analysis of amyloid A protein. In Amyloid and Amyloidosis 1988, Ed. by Isobe, T., Araki, S., Uchino, F., Kito, S., and Tsubura, E., Plenum Press, New York, 1989, p. 211-216.

3b. Kluve-Beckerman, B., Dwulet, F.E., and Benson, M.D.: Serum amyloid A: Characterization of three cDNAs in one individual and complete structure of their corresponding protein products. In Amyloid and Amyloidosis 1988, Ed. by Isobe, T., Araki, S., Uchino, F., Kito, S., and Tsubura, E., Plenum Press, New York, 1989, p. 265-270.

4b. Kluve-Beckerman, B., Brinckerhoff, C. and Benson, M.D.: Sequence analysis of a third human SAA gene. InAmyloid and Amyloidosis 1990, Ed. by Natvig, J.B., Forre, O., Husby, G., Husebekk, A., Skogen, B., Sletten, K., and Westermark, P., Kluwer Academic Publishers, Dordrecht/Boston/London, 1991, p. 24-27. 
5b. Harats, N., DiBartola, S.P., Benson, M.D. and Kluve-Beckerman, B.: Abyssinian cat model of AA amyloidosis: SAA gene analysis, In Amyloid and Amyloidosis 1990, Ed. by Natvig, J.B., Forre, O., Husby, G., Husebekk, A., Skogen, B., Sletten, K., and Westermark, P., Kluwer Academic Publishers, Dordrecht/Boston/London, 1991, p. 32-35.

6b. Kluve-Beckerman, B., Liepnieks, J. and Benson, M.D.: Differential regulation of human serum amyloid A isoforms. In Amyloid and Amyloidosis 1990, Ed. by Natvig, J.B., Forre, O., Husby, G., Husebekk, A., Skogen, B., Sletten, K., and Westermark, P., Kluwer Academic Publishers, Dordrecht/Boston/London, 1991, p. 125-128.

7b. Liepnieks, J.J., Leagre, C., Kluve-Beckerman, B. and Benson, M.D.: Predominance of one SAA isotype (SAAI) in human reactive amyloid. In Amyloid and Amyloidosis 1990, Ed. by Natvig, J.B., Forre, O., Husby, G., Husebekk, A., Skogen, B., Sletten, K., and Westermark, P., Kluwer Academic Publishers, Dordrecht/Boston/London, 1991, p. 511-514.

8b. Kluve-Beckerman, B., Song, M., Benson, M.D., and Liepnieks, J.J.: Baculovirus-infected insect cells as a source of isotypically pure, radiolabeled murine SAA2. In Amyloid and Amyloidosis, 1993. Ed. by Kisilevsky, R., Benson, M.D., Frangione, B., Gauldie, J., Muckle, T., and Young, I., Parthenon Publishing, Pearl River, NY, 1994, p. 38-40.

9b. Yamada, T., Kluve-Beckerman, B., Benson, M.D., and Liepnieks, J.J.: Efficient production of recombinant human serum amyloid A proteins. In Amyloid and Amyloidosis, 1993. Ed. by Kisilevsky, R., Benson, M.D., Frangione, B., Gauldie, J., Muckle, T., and Young, I., Parthenon Publishing, Pearl River, NY, p. 128-130.

10b. Benson, M.D., Kluve-Beckerman, B., Liepnieks, J.J., Murrell, J.R., Hanes, D., and Uemichi, T.: Metabolism of amyloid proteins. In The Nature and Origin of Amyloid Fibrils. Ciba Foundation Symposium 199. Wiley, Chichester, 1996, p. 104-118.